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KMID : 0616120220530010042
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Korean Journal of Pharmacognosy
2022 Volume.53 No. 1 p.42 ~ p.48
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Inhibitory Effect of RANKL-Induced Osteoclast Differentiation and ROS Generation by Sphaerotylus antarcticus Extract
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Kim Eun-Nam
Kang Da-Yeun
Nguyen Minh Trang
Lee Jun-Hyuck
Ko Young-Wook
Kim Sang-Hee
Na Min-Kyun
Jeong Gil-Saeng
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Abstract
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In order to maintain bone homeostasis, it is necessary to balance bone resorption and remodeling through the differentiation of osteoclasts that absorb old bone and osteoblasts that form new bone. However, bone resorption due to excessive osteoclast differentiation is a major cause of osteoporosis and controlling excessive osteoclast differentiation has been known as a treatment strategy for osteoporosis. Therefore, in this study, the effect of an ethanol extract of Sphaerotylus antarcticus Kirkpatrick, 1907 (SAE), polar-derived sponge with unknown biological activity, on the osteoclast differentiation process of RANKL-induced RAW264.7 cells and the generated ROS was evaluated. In the study results, SAE down-regulated the formation and function of RANKL-induced osteoclasts and osteoclast differentiation specific proteins, genes in a concentration-dependent manner. In addition, it was possible to confirm the result of restoring the lost antioxidant enzyme along with down-regulation of ROS generated by RANKL. Therefore, in this study, we propose the possibility of SAE as a potential regulator of osteoporosis due to excessive osteoclast differentiation and report the biological value of the diversity of marine-derived natural products by identifying the first biological activity against SAE that is not yet known.
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KEYWORD
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S. antarcticus, RANKL, RAW264.7, Osteoclast, Osteoporosis, ROS
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